Amy K. Schmid, Assistant Professor  

Amy K. Schmid

Although life science research has entered the post-genomic era, we still understand little about the diversity of microbial life on earth. Information is particularly lacking on microbial extremophiles, which thrive at the limits of life. Extremophiles can be found in deep-sea hydrothermal vents under high pressure and temperature, saturated salt lakes, and polar icecaps. Many of these organisms are members of the third domain of life, the archaea. How do these microorganisms cope with an extreme and changing environment? How do they alter their genetic programs and metabolic pathways to adapt and survive changes in their unique habitats on earth? Central to this process are gene regulatory networks (GRNs) composed of groups of regulatory proteins that switch genes on and off in response to environmental stimuli. Upon sensing a change in the environment, the GRN increases the production of genes encoding proteins that repair damage, restore the cell to a healthy state and prepare for future stress. The organism studied in the current research, called Halobacterium salinarum, thrives in high salt environments. The long-term aim of our work is to determine the underlying mechanisms by which regulatory factors interact in the GRN of H. salinarum enable survival during environmental perturbations. We are using a systems biology approach, which combines cutting-edge high throughput experimental techniques with computational or statistical modeling. Research toward these goals will lay the foundation for rational re-engineering of cellular physiology for desired outcomes such as targeted industrial, environmental and medical purposes.

Education:
Ph.D., University of Washington, 2004
B.S., Marquette University, 1997

Office Location: 125 Science Dr, French Family Science Center 4105, Durham, NC 27708
Office Phone: (919) 613-4464
Email Address: amy.schmid@duke.edu
Web Page: http://www.biology.duke.edu/schmidlab
Additional Web Page: http://www.biology.duke.edu/schmidlab

Specialties:
Genomics
Cell and Molecular Biology

Research Categories: Systems biology of archaea: understanding regulatory network responses to environmental extremes

Research Description: Although life science research has entered the post-genomic era, we still understand little about the diversity of microbial life on earth. Information is particularly lacking on microbial extremophiles, which thrive at the limits of life. Extremophiles can be found in deep-sea hydrothermal vents under high pressure and temperature, saturated salt lakes, and polar icecaps. Many of these organisms are members of the third domain of life, the archaea. How do these microorganisms cope with an extreme and changing environment? How do they alter their genetic programs and metabolic pathways to adapt and survive changes in their unique habitats on earth? Central to this process are gene regulatory networks (GRNs) composed of groups of regulatory proteins that switch genes on and off in response to environmental stimuli. Upon sensing a change in the environment, the GRN increases the production of genes encoding proteins that repair damage, restore the cell to a healthy state and prepare for future stress. The organism studied in the current research, called Halobacterium salinarum, thrives in high salt environments. The long-term aim of our work is to determine the underlying mechanisms by which regulatory factors interact in the GRN of H. salinarum enable survival during environmental perturbations. We are using a systems biology approach, which combines cutting-edge high throughput experimental techniques with computational or statistical modeling. Research toward these goals will lay the foundation for rational re-engineering of cellular physiology for desired outcomes such as targeted industrial, environmental and medical purposes.

Recent Publications   (More Publications)   (search)

  1. CL Darnell and AK Schmid, Systems biology approaches to defining transcription regulatory networks in halophilic archaea., Methods (San Diego, Calif.), vol. 86 (September, 2015), pp. 102-114 [doi]  [abs].
  2. PD Tonner, AM Pittman, JG Gulli, K Sharma and AK Schmid, A regulatory hierarchy controls the dynamic transcriptional response to extreme oxidative stress in archaea., PLoS genetics, vol. 11 no. 1 (January, 2015), pp. e1004912 [doi]  [abs].
  3. AJ Kulp, B Sun, T Ai, AJ Manning, N Orench-Rivera, AK Schmid and MJ Kuehn, Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli., PloS one, vol. 10 no. 9 (January, Submitted, 2015), pp. e0139200 [DukeSpace], [doi]  [abs].
  4. KA Dulmage, H Todor and AK Schmid, Growth-Phase-Specific Modulation of Cell Morphology and Gene Expression by an Archaeal Histone Protein., mBio, vol. 6 no. 5 (January, 2015), pp. e00649-e00615 [doi]  [abs].
  5. H Todor, J Gooding, OR Ilkayeva and AK Schmid, Dynamic Metabolite Profiling in an Archaeon Connects Transcriptional Regulation to Metabolic Consequences., PloS one, vol. 10 no. 8 (January, 2015), pp. e0135693 [doi]  [abs].

Curriculum Vitae