Publications [#199886] of Amy K Schmid
search .Papers Submitted
- Kriti Sharma, Nicolas Gillum, J. Lomax Boyd, Amy K. Schmid, RosR is a haloarchaeal-specific transcription factor required for the response to extreme oxidative stress in Halobacterium salinarum,
Journal of Biological Chemistry
(December, 2011) .
(last updated on 2011/12/17)Abstract:
Previous work has shown that the hypersaline-adapted archaeon, Halobacterium salinarum NRC-1, dynamically responds to oxidative stress, implicating a complex gene regulatory network. However, the molecular function of transcription regulatory proteins involved in this response remains unknown. Here we have leveraged the existing gene regulatory network and systems biology data for H. salinarum to identify a novel winged helix-turn-helix transcription factor, VNG0258H, which we have named RosR. This protein appears to be unique to the haloarchaea at the primary sequence level. Quantitative growth assays in a deletion mutant strain implicate RosR in extreme oxidative stress resistance. According to time course gene expression analyses, this transcription factor is required for the appropriate dynamic response of nearly 200 genes to reactive oxygen species damage from paraquat and hydrogen peroxide. These genes are predicted to function in repair of oxidative damage to proteins and DNA. in vivo DNA binding assays (ChIP-qPCR) demonstrate that RosR binds DNA to mediate gene regulation. Together these results suggest that RosR is a novel archaeal transcription regulatory protein that regulates gene expression to enable adaptation to the extremely oxidative, hypersaline niche of H. salinarum.
Duke Biology Box 90338 Durham, NC 27708 Phone: 919-660-7372 Fax: 919-660-7293