Nicolas Buchler, Assistant Professor
Our lab is interested in the systems biology and evolution of epigenetic switches (bistability) and clocks (oscillators) in gene regulatory networks, two functions that are essential for proper patterning, cell proliferation, and cellular differentiation in biological systems. We take an interdisciplinary approach (experiment and theory; biology and physics; systems and synthetic biology) to understand the diverse molecular and evolutionary mechanisms by which bistability and oscillation have evolved in gene networks. Our model organism is budding yeast, one of the best eukaryotes for a systems biology approach to study the evolution of gene networks. We measure and perturb the in vivo dynamics of gene networks using flow cytometry, time-lapse fluorescence microscopy, and yeast molecular genetics. We further combine this quantitative biology approach with comparative genomics to glean how novel dynamics and function have evolved in yeast gene networks.
PhD, University of Michigan, 2001
BS, University of California, San Diego, 1995
Cell and Molecular Biology
Research Categories: Molecular mechanisms and the evolution of switches and oscillators in gene networks; systems biology; comparative genomics
- M Zhou, W Wang, S Karapetyan, M Mwimba, J Marqués, NE Buchler, X Dong, Redox rhythm reinforces the circadian clock to gate immune response, Nature, vol. 523 (June, 2015), pp. 472-476 [doi] [abs].
- Y Tanouchi, A Pai, H Park, S Huang, R Stamatov, NE Buchler, L You, A noisy linear map underlies oscillations in cell size and gene expression in bacteria., Nature, vol. 523 (June, 2015), pp. 357-360 [html], [doi] [abs].
- A Mazo-Vargas, H Park, M Aydin, NE Buchler, Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy, Molecular Biology of the Cell, vol. 25 no. 22 (November, 2014), pp. 3699-708 [long], [doi] [abs].
- FR Cross, NE Buchler, JM Skotheim, Evolution of networks and sequences in eukaryotic cell cycle control., Philosophical Transactions of the Royal Society of London. Series B, Biological sciences, vol. 366 no. 1584 (December, 2011), pp. 3532-44 [doi] [abs].
- NE Buchler, FR Cross, Protein sequestration generates a flexible ultrasensitive response in a genetic network., Molecular Systems Biology, vol. 5 (2009), pp. 272 [doi] [abs].
- NE Buchler, M Louis, Molecular titration and ultrasensitivity in regulatory networks., Journal of Molecular Biology, vol. 384 no. 5 (December, 2008), pp. 1106-19 [doi] [abs].