David R. McClay, Arthur S. Pearse Professor  

David R. McClay

We ask how the embryo works. Prior to morphogenesis the embryo specifies each cell through transcriptional regulation and signaling. Our research builds gene regulatory networks to understand how that early specification works. We then ask how this specification programs cells for their morphogenetic movements at gastrulation, and how the cells deploy patterning information. Current projects examine 1) novel signal transduction mechanisms that establish and maintain embryonic boundaries mold the embryo at gastrulation; 2) specification of primary mesenchyme cells in such a way that they are prepared to execute an epithelial-mesenchymal transition, and then study mechanistically the regulation of that transition; 3) the specification of endoderm necessary for invagination of the archenteron; 4) formation of the oral/aboral ectoderm and the means by which patterning information is distributed three dimensionally around the embryo. That information is necessary for patterning and inducing skeletogenesis. Other projects examine neural tube folding with the goal of identifying genes associated with neural tube defects. Finally, a large current effort in systems biology is being expended with the goal of enlarging our knowledge of early networks and how they interact.

Education:
Ph.D., University of North Carolina at Chapel Hill, 1971
M.S., University of Vermont, 1965
B.S., Pennsylvania State University, 1963
BS Zoology, Penn State University, 1963

Office Location: 4102 French Science Center, Science Dr., Durham, NC 27708
Office Phone: (919) 613-8188
Email Address: dmcclay@duke.edu

Specialties:
Cell and Molecular Biology
Developmental Biology

Research Categories: Developmental Biology

Research Description: We ask how the embryo works. Prior to morphogenesis the embryo specifies each cell through transcriptional regulation and signaling. Our research builds gene regulatory networks to understand how that early specification works. We then ask how this specification programs cells for their morphogenetic movements at gastrulation, and how the cells deploy patterning information. Current projects examine 1) novel signal transduction mechanisms that establish and maintain embryonic boundaries mold the embryo at gastrulation; 2) specification of primary mesenchyme cells in such a way that they are prepared to execute an epithelial-mesenchymal transition, and then study mechanistically the regulation of that transition; 3) the specification of endoderm necessary for invagination of the archenteron; 4) formation of the oral/aboral ectoderm and the means by which patterning information is distributed three dimensionally around the embryo. That information is necessary for patterning and inducing skeletogenesis. Other projects examine neural tube folding with the goal of identifying genes associated with neural tube defects. Finally, a large current effort in systems biology is being expended with the goal of enlarging our knowledge of early networks and how they interact.

Representative Publications   (More Publications)   (search)

  1. Range, R; Glenn, T; McClay, DR, Lv-Numb promotes Notch-mediated specification of secondary mesenchyme cells in the sea urchin embryo, Development, vol. 135 no. 14 (December, 2008), pp. 2445-2454 [18550713], [doi]  [abs].
  2. Byrum, CA; Walton, KD; Robertson, AJ; Carbonneau, S; Thomason, RT; Coffman, JA; McClay, DR, Protein tyrosine and serine-threonine phosphatases in the sea urchin, Strongylocentrotus purpuratus: identification and potential functions., Developmental Biology, vol. 300 no. 1 (December, 2006), pp. 194-218 [17087928], [doi]  [abs].
  3. Lapraz, F; R├Âttinger, E; Duboc, V; Range, R; Duloquin, L; Walton, K; Wu, SY; Bradham, C; Loza, MA; Hibino, T; Wilson, K; Poustka, A; McClay, D; Angerer, L; Gache, C; Lepage, T, RTK and TGF-╬▓ signaling pathways genes in the sea urchin genome, Developmental Biology, vol. 300 no. 1 (2006), pp. 132-152 [doi]  [abs].
  4. Croce, JC; Wu, SY; Byrum, C; Xu, R; Duloquin, L; Wikramanayake, AH; Gache, C; McClay, DR, A genome-wide survey of the evolutionarily conserved Wnt pathways in the sea urchin Strongylocentrotus purpuratus., Developmental Biology, vol. 300 no. 1 (December, 2006), pp. 121-131 [17069790], [doi]  [abs].
  5. Walton, KD; Croce, JC; Glenn, TD; Wu, SY; McClay, DR, Genomics and expression profiles of the Hedgehog and Notch signaling pathways in sea urchin development., Developmental Biology, vol. 300 no. 1 (December, 2006), pp. 153-164 [17067570], [doi]  [abs].
  6. Bradham, CA; Foltz, KR; Beane, WS; Arnone, MI; Rizzo, F; Coffman, JA; Mushegian, A; Goel, M; Morales, J; Geneviere, AM; Lapraz, F; Robertson, AJ; Kelkar, H; Loza-Coll, M; Townley, IK; Raisch, M; Roux, MM; Lepage, T; Gache, C; McClay, DR; Manning, G, The sea urchin kinome: a first look., Developmental Biology, vol. 300 no. 1 (December, 2006), pp. 180-193 [17027740], [doi]  [abs].
  7. Beane, WS; Voronina, E; Wessel, GM; McClay, DR, Lineage-specific expansions provide genomic complexity among sea urchin GTPases., Developmental Biology, vol. 300 no. 1 (December, 2006), pp. 165-179 [17014838], [doi]  [abs].
  8. Robertson, AJ; Croce, J; Carbonneau, S; Voronina, E; Miranda, E; McClay, DR; Coffman, JA, The genomic underpinnings of apoptosis in Strongylocentrotus purpuratus, Developmental Biology, vol. 300 no. 1 (2006), pp. 321-334 [doi]  [abs].
  9. Sea Urchin Genome Sequencing Consortium, ; Sodergren, E; Weinstock, GM; Davidson, EH; Cameron, RA; Gibbs, RA; Angerer, RC; Angerer, LM; Arnone, MI; Burgess, DR; Burke, RD; Coffman, JA; Dean, M; Elphick, MR; Ettensohn, CA; Foltz, KR; Hamdoun, A; Hynes, RO; Klein, WH; Marzluff, W; McClay, DR; Morris, RL; Mushegian, A; Rast, JP; Smith, LC; Thorndyke, MC; Vacquier, VD; Wessel, GM; Wray, G; Zhang, L; Elsik, CG; Ermolaeva, O; Hlavina, W; Hofmann, G; Kitts, P; Landrum, MJ; Mackey, AJ; Maglott, D et al., The genome of the sea urchin Strongylocentrotus purpuratus., Science, vol. 314 no. 5801 (November, 2006), pp. 941-952 [17095691], [doi]  [abs].
  10. Oliveri, P; Walton, KD; Davidson, EH; McClay, DR, Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo, Development (Cambridge), vol. 133 no. 21 (2006), pp. 4173-4181 [doi]  [abs].
  11. Croce, JC; McClay, DR, The canonical Wnt pathway in embryonic axis polarity., Seminars in Cell and Developmental Biology, vol. 17 no. 2 (April, 2006), pp. 168-174 [16714126], [doi]  [abs].
  12. Bradham, C; McClay, DR, p38 MAPK in development and cancer., Cell Cycle, vol. 5 no. 8 (April, 2006), pp. 824-828 [16627995], [doi]  [abs].
  13. Beane, WS; Gross, JM; McClay, DR, RhoA regulates initiation of invagination, but not convergent extension, during sea urchin gastrulation., Developmental Biology, vol. 292 no. 1 (April, 2006), pp. 213-225 [16458878], [doi]  [abs].
  14. Croce, J; Duloquin, L; Lhomond, G; McClay, DR; Gache, C, Frizzled5/8 is required in secondary mesenchyme cells to initiate archenteron invagination during sea urchin development, Development, vol. 133 no. 3 (2006), pp. 547-557 [doi]  [abs].
  15. Bradham, CA; McClay, DR, p38 MAPK is essential for secondary axis specification and patterning in sea urchin embryos., Development (Cambridge), vol. 133 no. 1 (January, 2006), pp. 21-32 [16319119], [doi]  [abs].