Publications [#17345] of Steve Haase
search PubMed.Papers Published
- S. B. Haase and S. I. Reed, Evidence that a free-running oscillator drives G1 events in the budding yeast cell cycle,
Nature no. 401
(1999),
pp. 394-397 .
(last updated on 2003/12/29)Author's Comments:
this work was reviewed in: M.R. Roussel. An autonomous cell- cycle oscillator involved in the coordination of G1 events. Bioessays. 2000 Jan;22(1):3-5.Abstract:
In yeast and somatic cells, mechanisms ensure cell-cycle events are initiated only when preceding events have been completed. In contrast, interruption of specific cell-cycle processes in early embryonic cells of many organisms does not affect the timing of subsequent events, indicating that cell-cycle events are triggered by a free-running cell-cycle oscillator. Here we present evidence for an independent cell-cycle oscillator in the budding yeast Saccharomyces cerevisiae. We observed periodic activation of events normally restricted to the G1 phase of the cell cycle, in cells lacking mitotic cyclin-dependent kinase activities that are essential for cell-cycle progression. As in embryonic cells, G1 events cycled on schedule, in the absence of S phase or mitosis, with a period similar to the cell-cycle time of wild-type cells. Oscillations of similar periodicity were observed in cells responding to mating pheromone in the absence of G1 cyclin (Cln)- and mitotic cyclin (Clb)-associated kinase activity, indicating that the oscillator may function independently of cyclin-dependent kinase dynamics. We also show that Clb-associated kinase activity is essential for ensuring dependencies by preventing the initiation of new G1 events when cell-cycle progression is delayed.
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