Publications [#262764] of Tuan Vo-Dinh

Papers Published

1. Allain, LR; Vo-Dinh, T, Surface-enhanced Raman scattering detection of the breast cancer susceptibility gene BRCA1 using a silver-coated microarray platform, Analytica Chimica Acta, vol. 469 no. 1 (September, 2002), pp. 149-154, Elsevier BV, ISSN 0003-2670 [doi]
   (last updated on 2024/04/24)

   Abstract:
   Surface-enhanced Raman scattering (SERS) spectroscopy was used to monitor DNA hybridization of a fragment of the BRCA1 breast cancer susceptibility gene on modified silver surfaces. Rhodamine B was covalently attached to a 5′-amino-labeled oligonucleotide sequence (23 mer) through a succinimidyl ester intermediate in methanol. The silver surfaces were prepared by depositing a discontinuous layer (9.0nm) of silver onto glass slides, which had been etched with HF to form a microwell platform, and subsequently modified with a monolayer of mercaptoundecanoic acid. The complementary probe was covalently attached to the silver surfaces using a succinimidyl ester intermediate in acetonitrile. The silver island substrate allows a very large enhancement of the Raman signal of the DNA-Rhodamine B, and clear distinction between hybridized samples and controls on a microwell array sampling platform. © 2002 Elsevier Science B.V. All rights reserved.