Publications [#262879] of Tuan Vo-Dinh

Papers Published

1. Tromberg, BJ; Sepaniak, MJ; Vo-Dinh, T; Griffin, GD, Fiber-optic chemical sensors for competitive binding fluoroimmunoassay., Analytical chemistry, vol. 59 no. 8 (April, 1987), pp. 1226-1230, ISSN 0003-2700 [3296852], [doi]
   (last updated on 2024/04/18)

   Abstract:
   This paper describes the development of a fiber-optic chemical sensor based on the principle of competitive-binding fluorescence immunoassay. Rabbit immunoglobin G (IgG) is covalently immobilized on the distal sensing tip of a quartz optical fiber. The sensor is exposed to fluorescein isothiocyanate (FITC) labeled and unlabeled anti-rabbit IgG. The 488-nm line of an argon-ion laser provides excitation of sensor-bound analyte. This results in fluorescence emission at the optical fiber's sensing tip. Sensor response is inversely proportional to the amount of unlabeled anti-IgG in the sample. Limits of detection (LOD) vary with incubation time, sample size, and measurement conditions.

   Keywords:
   BIOCHEMISTRY - Sensors;FIBER OPTICS - Applications;ANTIGENS - Sensors;