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Publications [#263011] of Tuan Vo-Dinh

Papers Published

  1. Song, JM; Griffin, GD; Vo-Dinh, T, Application of an integrated microchip system with capillary array electrophoresis to optimization of enzymatic reactions, Analytica Chimica Acta, vol. 487 no. 1 (July, 2003), pp. 75-82, Elsevier BV [S0003-2670(03)00515-4], [doi]
    (last updated on 2024/03/28)

    Abstract:
    In this work, a combination of complementary metal-oxide semiconductor (CMOS) microchip system with capillary array electrophoresis (CAE) is demonstrated as a system for optimizing conditions for enzymatic reaction. Dimethylacridinone (DDAO)-phosphate substrate and alkaline phosphatase conjugate were selected for the enzymatic reaction, which was applicable to the enzyme-linked immunosorbent assay (ELISA) technique. Laser-induced fluorometry with a miniature semiconductor laser was used to detect the enzymatic products. The speed of the enzymatic reaction between the DDAO-phosphate and the alkaline phosphatase conjugate was investigated as a function of reaction time. The microchip-CAE detection system could determine the pH condition and the concentration of enzyme that are suitable for rapid and low-cost analysis. This result shows the feasibility of using the microchip-CAE system for application to miniaturized screening systems. © 2003 Elsevier Science B.V. All rights reserved.

    Keywords:
    Enzyme kinetics;Electrophoresis;Bioassay;Semiconductor lasers;pH effects;Phosphates;Computer aided engineering;


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