Publications [#263018] of Tuan Vo-Dinh

Papers Published

1. Song, JM; Culha, M; Kasili, PM; Griffin, GD; Vo-Dinh, T, A compact CMOS biochip immunosensor towards the detection of a single bacteria., Biosensors & bioelectronics, vol. 20 no. 11 (May, 2005), pp. 2203-2209, ISSN 0956-5663 [15797317], [doi]
   (last updated on 2024/04/18)

   Abstract:
   Recent use of biological warfare (BW) agents has led to a growing interest in the rapid and sensitive detection of pathogens. Therefore, the development of field-usable detection devices for sensitive and selective detection of BW agents is an important issue. In this work, we report a portable biochip system based on complementary metal oxide semiconductor (CMOS) technology that has great potential as a device for single-bacteria detection. The possibility of single-bacteria detection is reported using an immunoassay coupled to laser-induced fluorescence (LIF) detection. Bacillus globigii spores, which are a surrogate species for B. anthracis spores, were used as the test sample. Enzymatic amplification following immunocomplex formation allowed remarkably sensitive detection of B. globigii spores, and could preclude a complicated optical and instrumental system usually required for high-sensitive detection. Atomic force microscopy (AFM) was employed to investigate whether B. globigii spores detected in the portable biochip system exist in single-cell or multicellular form. It was found that B. globigii spores mostly exist in multicellular form with a small minority of single-cell form. The results showed that the portable biochip system has great potential as a device for single-particle or possibly even single-organism detection.

   Keywords:
   Bacteria;CMOS integrated circuits;Microprocessor chips;Immunology;Pathology;Fluorescence;Complexation;Atomic force microscopy;