Publications [#232717] of Katherine J. Franz

Journal Articles

  1. Wöhnert, J; Franz, KJ; Nitz, M; Imperiali, B; Schwalbe, H, "Protein alignment by a coexpressed lanthanide-binding tag for the measurement of residual dipolar couplings.", Journal of the American Chemical SocietyNovember,, 2003, 125(44), 13338-13339 [doi].
    (last updated on 2023/01/01)

    Abstract:
    A protein fusion construct of human ubiquitin with an N-terminal lanthanide binding tag (LBT) enables observation of long-range orientational restraints in solution NMR from residual dipolar couplings (RDCs) due to paramagnetic alignment of the protein. The paramagnetic lanthanide ions Tb3+, Dy3+, and Tm3+ are shown to bind to the LBT and induce different alignment tensors, in agreement with theory. RDCs, measured relative to the diamagnetic Lu3+, range from -7.6 to 5.5 Hz for Tb3+ and -6.6 to 6.1 Hz for Dy3+, while an opposite alignment tensor is observed for Tm3+ (4.5 to -2.9 Hz) at 800 MHz. Experimental RDCs are in excellent agreement with those predicted on the basis of the X-ray structure of the protein.