Publications of Michael C. Fitzgerald

Papers Accepted

  1. Cisneros, G.A., Wang, M., Silinski, P., Fitzgerald, M.C., and Yang, W., "Theoretical and Experimental Determination on Two Subtrates Turned Over by 4-Oxalocrotonate Tautomerase", Journal of Physical Chemistry, 2005.
  2. Wang, M., Wales, T.E., and Fitzgerald, M.C., "The Thermodynamics of Backbone-Backbone Hydrogen Bonds are Conserved ina Protein Fold", Proceedings of the National Academy of Sciences, USA, 2005 (In Press.).

Papers Submitted

  1. West, G., Tang, L., and Fitzgerald, M.C., "Thermodynamic Analysis of Protein Stability and Ligand Binding using a Chemical Modification- and Mass Spectrometry-Based Strategy", Analytical Chemistry, 2007.

Journal Articles

  1. Frankel, B.A., Tong, Y., Bentley, M.L., Fitzgerald, M.C., and McCafferty, D.G., "Analysis of Active Site Residues in the Staphylococcus aureus Transpeptidase SrtA Mutational", Biochemistry, 2007(46), 7269-7278.
  2. Williams, J.C., Roulhac, P.L., Roy, A.G., Vallee, R.B., Fitzgerald, M.C., and Hendrickson, W.A., "Structural and Thermodynamic Characterization of a Cytoplasmic Dynein Light Chain-Intermediate Chain Complex.", Proceedings of the National Academy of Sciences, USA,, 2007(104), 10028-10033.
  3. Tang, L., Hopper, E.D., Tong, Y., Sadowsky, J.D., Peterson, K.J., Gellman, S.H., and Fitzgerald, M.C., "H/D Exchange- and Mass Spectrometry-Based Strategy for the Thermodynamic Analysis of Protein-Ligand Binding", Analytical Chemistry, 2007(79), 5869-5877.
  4. Tang, L., Roulhac, P.L., and Fitzgerald, M.C., "H/D Exchange and Mass Spectrometry-Based Method for Biophysical Analysis of Multidomain Proteins at the Domain Level", Analytical Chemistry, 2007(79), 8728-8739.
  5. Dai, S.Y., Gardner, M.W., and Fitzgerald, M.C., "An Improved Protocol for the SUPREX (Stability of Unpurified Proteins from Rates of H/D Exchange) Analysis of Some Proteins", Analytical Chemistry, 2005, 77, 693-697.
  6. Tong, Y., Wuebbens, M.M., Rajagopalan, K.V., and Fitzgerald, M.C., "Thermodynamic Analysis of Subunit Interactions in Escherichia coli Molybdopterin Synthase", Biochemistry, 2005, 44, 2595-2601.
  7. Lin, S., Haney, R.P., Campa, M.J., Fitzgerald, M.C., and Patz, E.F., "Characterising Phase Variations in MALDI-TOF Data and Correcting them by Peak Alignment", Cancer Informatics, 2005, 1(1), 32-40.
  8. Dai, S.Y.; Gardner, M.W.; and Fitzgerald, M.C., "SUPREX (Stability of Unpurified Proteins from Rates of H/D Exchange) Analysis of Some Proteins", Analytical Chemistry, December 10, 2004 (Published on the Web.).
  9. Cisneros, G.A.; Wang, M.; Silinski, P.; Fitzgerald, M.C., and Yang, W., ""The Protein Backbon Makes Important Contributions to 4-Oxalocrotonate Tautomerase Enzyme Catalysis: Understanding from Theory and Experiment"", Biochemistry, 2004, 43, 6885-6892.
  10. Wales, T.E.; Richardson, J.S.; and Fitzgerald, M.C., ""Facile Chemical Synthesis and Equilibrium Unfolding Properties of CopG"", Protein Science, 2004, 13, 1918-1926.
  11. Yang, X.; Wang, M. ; and Fitzgerald, M.C., ""Analysis of Protein Folding and Function using Backbone Modified Proteins"", Bioorganic Chemistry, 2004, 32, 438-449.
  12. Wang, M.Z.; Shetty, J.T.; Howard, B.A.; Campa, M.J.; Patz, E.F. and Fitzgerald, M.C., ""Thermodynamic Analysis of Cyclosporin A Binding to Cyclophilin A in a Lung Tumor Tissue Lysate"", Analytical Chemistry, 2004, 76, 4343-4348.
  13. Howard, B.A.; Zheng, Z.; Campa, M.J.; Wang, M.Z., Sharma, A.; Haura, E.; Herndon, J.E.; Fitzgerald, M.C.; Bepler, G.; and Patz, E.F., ""Translating Biomarkers into Clinical Practice: Prognostic Implications of Cyclophilin A and Macrophage Migration Inhibitory Factor Identified from Protein Expression Profiles in Non-Small Cell Lung Cancer"", Lung Cancer, 2004, 46, 313-323.
  14. Roulhac, P.L.; Powell, K.D.; Dhungana, S.; Weaver, K.D.; Mietzner, T.A.; Crumbliss, A.L.; and Fitzgerald, M.C., "SUPREX (Stability of Unpurified Proteins from Rates of H/D Exchange) Analysis of the Thermodynamics of Synergistic Anion Binding by Ferric Binding Protein (FbpA), a Bacterial Transferrin", Biochemistry, 2004, 43, 15767-15774.
  15. Ma, L. and Fitzgerald, M.C., "Thermodynamic Analysis of Protein-DNA Interactions using a New H/D Exchange- and Mass Spectrometry-Based Method", Chemistry & Biology, December, 2003, 10.
  16. Campa, M.J., Wang, M.Z., Howard, B., Fitzgerald, M.C., and Patz, E.F., "Protein Expression Profiling Identifies MIF and Cyclophilin A as Potential Molecular Targets in Non-Small Cell Lung Cancer", Cancer Research, 2003, 63(7), 1652-1656.
  17. Powell, K.D. and Fitzgerald, M.C., "The Accuracy and Precisi9n of a New H/D Exchange- and Mass Spectrometry-Based Technique for Measuring the Thermodynamic Properties of Protein-Peptide Complexes", Biochemistry, 2003, 42, 4962-4970.
  18. Silinski, P. and Fitzgerald, M.C., "Comparative Analysis of Two Different Amide-to-Ester Bond Mutations in the ß-Sheet of 4-Oxalocrotonate Tautomerase", Biochemistry, 2003, 42(21), 6620-6630.
  19. Wang, M.Z., Howard, B., Campa, M.J., Patz, E.F., Fitzgerald, M.C., "Analysis of Human Serum Proteins by Liquid Phase Iso-Electric Focusing and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry", Proteomics, 2003, 3, 1661-1666.
  20. Howard, B.A., Wang, M.Z., Campa, M.J., Corro, C., Fitzgerald, M.C., and Patz, E.F., "Identification and Validation of a Potential Lung Cancer Serum Biomarker Detected by Matrix-Assisted Laser Desorption/Ionization-Time of Flight Spectral Analysis", Proteomics, 2003, 3, 1720-1724.
  21. Powell, K.D., Wang, M.Z., Silinski, P., Ma, L., Wales, T.E., Dai, S., Warner, A.H., Yang, Xiaoye, and Fitzgerald, M.C., "The Accuracy and Precision of a New H/D Exchange- and Mass Spectrometry-Based Technique for Measuring the Thermodynamic Stability of Proteins", Analytica Chimica Acta, 2003, 496, 225-232 (Invited Contribution for Young North American Faculty Special Issue.).
  22. Powell, K.D. Wales, T.E., and Fitzgerald, M.C., "Thermodynamic Stability Measurements on Multimeric Proteins Using a New H/D Exchange- and Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectromety-Based Method", Protein Science, 2002, 11, 841-851.
  23. Silinski, P. and Fitzgerald, M.C., "A Stable Dinner in the pH-Induced Equilibrium Unfolding of the Homo-Hexameric Enzyme 4-Oxalocrontonate Tautomerase (4-OT)", Biochemistry, 2002, 41(13), 4480-4491.
  24. Powell, K.D., Ghaemmaghami, S., Wang, M.Z., Liyuan, M., Oas, T.G., and Fitzgerald, M.C., "A General Mass Spectrometry-Based Assay for the Huantitation of Protein-Ligand Binding Interactions in Solution", Journal of the American Chemical Society, 2002, 124, 10256-10257.
  25. Wang, M. Z. and Fitzgerald, M.C., "A Solid Sample Preparation Method Reduces Signal Suppression Effects in the MALDI Analysis of Peptides.", Analytical Chemistry, 2001, 73(3), 625-631.
  26. Powell, K.D. and Fitzgerald, M.C., "High Sensitivity Measurements of Protein Stability by H/D Exchange and Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass spectrometry.", Analytical Chemistry, 2001, 73(14), 3300-3304.
  27. Moss, J.A., Silinski, P., and Fitzgerald, M.C., "Mapping Tertiary Interactions in Protein Folding Reactions: A Novel Mass Spectrometry- and Chemical Synthesis-Based Approach", Fresenius Journal of Analytical Chemistry, 2001, 369, 252-257 (Invited Contribution for Special North American Authors Issue.).
  28. Silinski, P., Allingham, M.J., and Fitzgerald, M.C., "The Guanidine-Induced Equilibrium Unfolding of a Homo-Hexameric Enzyme 4-Oxalocrotonate Tautomerase (4-OT)", Biochemistry, 2001, 40(14), 4493-4502.
  29. Whales, T.E. and Fitzgerald, M.C., "The Energetic Contribution of Backbone-Backbone Hydrogen Bonds to the Thermodynamic Stability of a Hyperstable P22 Arc Repressor Mutant", Journal of the American Chemical Society, 2001, 123, 7709-7710.
  30. Ghaemmaghami, S., Fitzgerald, M.C., and Oas, T.G., "A Quantitative, High-Throughput Screen for Protein Stability.", Proceedings of the National Academy of Sciences, 2000, 97(15), 8296-8301.
  31. Nakhle, B. M., Silinski, P., and Fitzgerald, M.C., "Identification of an Essential Backbone Amide Bond in the Folding and Stability of a Multimeric Enzyme", Journal of the American Chemical Society, 2000, 122(34), 8105-8111.
  32. Johnson, W.H., Czerwinski, R.M., Fitzgerald, M.C. and Whitman, C.P., "Inactivation of 4-Oxalocrotonate Tautomerase by 2-Oxo-3-pentynoate", Biochemistry, 1998, 36(50), 15724-15732.
  33. Stamps, S.L., Fitzgerald, M.C. and Whitman, C.P., "Characterization of the Role of the Amino-Terminal Proline in the Enzymatic Activity Catalyzed by Macrophage Migration Inhibitory Factor", Biochemistry, 1998, 37(28), 10195-10202.
  34. Winston, R.L. and Fitzgerald, M.C., "Concentration and Desalting of Protein Samples for Mass Spectrometry Analysis", Analytical Biochemistry, 1998, 262, 83-85.
  35. Beeston, R.F., Aldridge, W.S., Treadway, J.A., Fitzgerald, M.C., Degraff, B.A., and Stizel, S.E., "Synthesis, Characterization and Photochemical/Photophysical Properties of Ruthenium (II) Complexes with Hexadentate Bipyridine and phenanthroline Ligands", Inorganic Chemistry, 1998, 37(17), 4368-4379.
  36. Dawson, P.E., Fitzgerald, M.C., Muir, T.W. and Kent, S.B.H., "Methods for the Chemical Synthesis and Readout of Self-Encoded Arrays of Polypeptide Analogues", Journal of the American Chemical Society, 1997, 119, 7917-7927.
  37. Laco, G.S., Fitzgerald, M.C., Morris, G.M., Olson, A.J., Kent, S.B.H., and Elder, J.H., "Molecular Analysis of the Feline Immunodeficiency Virus Protease: Generation of a Novel Form of the Protease by Autoproteolysis and Construction of Cleavage Resistant Proteases", Journal of Virology, 1997, 71(7), 5505-5511.
  38. Carrasco, M.R., Fitzgerald, M.C., Oda, Y., and Kent, S.B.H., "Direct Monitoring of Organic Reactions on Polymeric Supports", Tetrahedron Letters, 1997, 38(36), 6331-6334.
  39. Fitzgerald, M.C., Laco, G.S., Elder, J.H., and Kent, S.B.H., "A Continuous Fluorometric Assay for the FIV Protease", Analytical Biochemistry, 1997, 254(2), 226-230.
  40. Winston, R.L. and Fitzgerald, M.C., "Mass Spectrometry as a Readout of Protein Structure and Function", Mass Spectrometry Reviews, 1997, 16, 165-179.
  41. Fitzgerald, M.C., Harris, K., Shevlin, C.G., and Siuzdak, G., "Direct Characterization of Solid Phase Resin-Bound Molecules by Mass Spectrometry", Bioorganic and Medicinal Chemistry Letters, 1996, 6(8), 979-982.
  42. Muir, T.M., Dawson, P.E., Fitzgerald, M.C. and Kent, S.B.H., "Probing the Chemical Basis of Binding Activity in an SH3 Domain by Protein Signature Analysis", Chemistry and Biology, 1996, 3(10), 817-825.
  43. Qian, X., Moris-Varas, F., Fitzgerald, M.C., and Wong, C.H., "C2-Symmetrical Tetrahydroxyazepenes as Inhibitors of Glyosidases and HIV/FIV Proteases", Bioorganic & Medicinal Chemistry, 1996, 4(12), 2055-2069.
  44. Fitzgerald, M.C. and Siuzdak, G., "Biochemical Mass Spectrometry: Worth the Weight?", Chemistry and Biology, 1996, 3, 707-715.
  45. Fitzgerald, M.C., Chernushevich, I., Standing, K.G., Whitman, C.P. and Kent, S.B.H., "Probing the Oligomeric Structure of an Enzyme by Electrospray Ionization Time-of-Flight Mass Spectrometry", Proceedings of the Natiopnal Academy of Sciences USA, 1996, 93, 6851-6856.
  46. Zhu, L., Fitzgerald, M.C., Parr, G.R. and Smith L.M., "Oligodeoxynucleotide Fragmentation in MALDI-TOF Mass Spectrometry Using 355 nm Radiation", Journal of the American Chemical Society, 1995, 117(22), 6048-6056.
  47. Fitzgerald, M.C., Chernushevich, I., Standing, K.G., Kent, S.B.H. and Whitman, C.P., "Total Chemical Synthesis and Catalytic Properties of the Enzyme Enantimers L- and D-4-Oxalocrotonate Tautomerase", Journal of the American Chemical Society, 1995, 117(45), 11075-11080.
  48. Fitzgerald, M.C. and Smith L.M., "Mass Spectrometry of Nucleic Acids: The Promise of Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry", Annual Review of Biophysics and Biomolecular Structure, 1995, 24, 117-140.
  49. Fitzgerald, M.C., Zhu, L. and Smith, L.M., "The Analysis of Mock DNA Sequencing Reactions Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry", Rapid Communications in Mass Spectrometry, 1993, 7, 895-897.
  50. Fitzgerald, M.C., Parr, G.R. and Smith, L.M., "Basic Matrices for the Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Proteins and Oligonucleotides", Analytical Chemistry, 1993, 65(22), 3204-3211.
  51. Fitzgerald, M.C., Skowron, P., Van Etten, J.L., Smith, L.M. and Mead, D.A., "Rapid Shotgun Cloning Utilizing the Two Base Recognition Endonuclease", Civil Nucleic Acids Research, 1992, 20(14), 3753-3762.
  52. Parr, G.R., Fitzgerald, M.C. and Smith, L.M., "Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Synthetic Oligodeoxyribonucleotides", Rapid Communications in Mass Spectrometry, 1992, 6, 369-372.
  53. Beeston, R.F., Larson, S.L., and Fitzgerald, M.C., "Ground- and Excited-State Properties of a Photostable Hemicage Ruthenium(II) Polypryidine Complex", Inorganic Chemistry, 1989, 28, 4187-4189.
  54. Whales, T.E. and Fitzgerald, M.C., "The Energetic Contribution of Backbone-Backbone Hydrogen Bonds to the Thermodynamic Stability of a Hyperstable P22 Arc Repressor Mutant.", Journal of the American Chemical Society, 123, 7709-7710.

Chapter in Book

  1. Fitzgerald, M.C., Dai, S., and Tong, Y., "SUPREX Analysis of Protein Folding and Function.", in Encyclopedia of Mass Spectrometry, edited by M.L. Gross and R.M. Caprioli , 2007, 6, 794-802.
  2. Fitzgerald, M.C., Tang, L., and Hopper, E.D., "Thermodynamic Analysis of Protein Folding and Ligand Binding by SUPREX", in Protein Mass Spectrometry, edited by J. Whitelegge , 2007, Elsevier, Inc. (In Press.).
  3. Winston, R.L. and Fitzgerald, M.C., "Combinatorial Chemistry and the Structure-Function Analysis of Proteins", in Peptide and Protein Drug Analysis, edited by Reid, R.E. , 2000, 101, 207-235, Marcel Dekker, Inc., New York.
  4. Fitzgerald, M.C. and Kent, S.B.H., "A General Approach to Deciphering How Proteins Work Using Synthetic Chemistry and Mass Spectrometry", in NATO ASI Series: New Methods for the Study of Molecular Aggregates, edited by Standing, K.G. and Ens, W. , 1998, Kluwer Academic Publishers, The Netherlands.
  5. Fitzgerald, M.C. and Kent, S.B.H., "Total Chemical Synthesis of Proteins", in Bioorganic Chemistry: Peptides and Proteins, edited by Hecht, S.M. , 1998, 65-99, Oxford University Press, New York.
  6. Muir, T.M., Dawson, P.E., Fitzgerald, M.C., and Kent, S.B.H., "Protein Signature Analysis: A Practical New Approach for Studying Structure-Activity Relationships in Peptide and Proteins", in Methods in Enzymology: Solid-Phase Peptide Synthesis, edited by Fields, G.B. , 1997, 289, 545-564, Academic Press, New York.