Publications [#304373] of Michael C. Fitzgerald

Journal Articles

  1. Dai, SY; Gardner, MW; Fitzgerald, MC, "Protocol for the thermodynamic analysis of some proteins using an H/D exchange- and mass spectrometry-based technique.", Analytical chemistryJanuary,, 2005, 77(2), 693-697 [15649073], [doi].
    (last updated on 2024/04/19)

    Abstract:
    SUPREX (stability of unpurified proteins from rates of H/D exchange) is a new H/D exchange- and mass spectrometry-based technique for the measurement of protein folding free energies (i.e., DeltaG values) and protein folding m values (i.e., deltaDeltaG/delta[denaturant]). Robust protocols for the acquisition and analysis of SUPREX data have been established and shown to be useful for the analysis of a number of different protein systems. Here we report on the SUPREX behavior of a special class of proteins that are not amenable to conventional SUPREX analyses using previously established protocols. This class of proteins includes protein systems that require an extended time to reach a folding/unfolding equilibrium in chemical denaturant-induced equilibrium unfolding experiments. As part of this work we use ubiquitin as a model system to highlight the complications that can arise in the conventional SUPREX analysis of such protein systems, and we describe a modified SUPREX protocol that can be used to eliminate these complications.