Publications [#30120] of Rytas Vilgalys
- TY James, SR Liou & R Vilgalys, The genetic structure and diversity of the A and B mating-type genes from the tropical oyster mushroom, Pleurotus djamor.,
Fungal Genet Biol, United States, vol. 41 no. 8
pp. 813-825, ISSN 1087-1845 [doi]
(last updated on 2005/04/06)
In most heterothallic mushroom species, inbreeding is avoided by an incompatibility system determined by two loci each with multiple alleles (the A and B mating-type loci). In this study we investigated the genetic structure of the mating-type loci in the tropical oyster mushroom Pleurotus djamor using both positional cloning and degenerate PCR methods. DNA sequences from genomic regions cosegregating with the mating-type loci of P. djamor revealed homeodomain transcription factors (A) and pheromone receptors (B), suggesting the genetic basis for mating-type determination in P. djamor is the same as in the model mushroom species. Three pheromone receptors were detected in a single homokaryotic isolate of P. djamor. Only one pair of homeodomain genes was detected in the A mating-type region. It is hypothesized that the A mating-type locus of P. djamor is comprised of only one homeodomain pair, which may explain the lower number of A mating-type alleles relative to other mushroom species.
Amino Acid Sequence • Chromosome Mapping • Gene Library • Genes, Fungal* • Genes, Mating Type, Fungal* • Genetic Variation* • Molecular Sequence Data • Phylogeny • Pleurotus • Receptors, Pheromone • Sequence Homology, Amino Acid • Transcription Factors • classification • genetics • genetics* • physiology