Publications [#30122] of Rytas Vilgalys
- TY James, U Kües, SA Rehner & R Vilgalys, Evolution of the gene encoding mitochondrial intermediate peptidase and its cosegregation with the A mating-type locus of mushroom fungi.,
Fungal Genet Biol, United States, vol. 41 no. 3
pp. 381-390, ISSN 1087-1845 [doi]
(last updated on 2005/04/06)
The high level of DNA polymorphism at the mating-type loci of mushroom fungi has made the cloning of mating-type genes difficult. As an alternative to strategies that employ sequence conservation, an approach utilizing conserved gene order could facilitate the cloning of A mating-type genes from mushroom fungi. It has been shown that a gene encoding a mitochondrial intermediate peptidase (MIP) is very close ( < 1 kbp) to the A mating-type locus of two model mushroom species. In this study, the cosegregation of MIP and the A mating-type locus was studied by genotyping progeny of seven additional mushroom species using PCR and genetic crosses. No evidence of any recombination between MIP and the A mating-type locus was detected among all seven species. Phylogenetic analysis of MIP sequences from diverse mushroom species agrees with the current organismal phylogeny, suggesting the sequences are generally orthologous.
Agaricales • Base Sequence • DNA Primers • DNA, Fungal • Genetic Variation • Metalloendopeptidases • Peptides • Phylogeny • classification • enzymology* • genetics • genetics* • isolation & purification