Publications [#174293] of G. Allan Johnson

Papers Published
  1. GA Johnson, TR Hansen, KJ Austin, EA Van Kirk, WJ Murdoch, Baculovirus-insect cell production of bioactive choriogonadotropin-immunoglobulin G heavy-chain fusion proteins in sheep., Biology of reproduction, vol. 52 no. 1 (January, 1995), pp. 68-73 .

    A hybrid cDNA encoding a fusion protein between the beta subunit of hCG (beta hCG) and constant domains of a mouse IgG heavy chain (CH1-3) was inserted into a baculovirus expression vector. Insect cells transfected with foreign DNA synthesized multimeric forms of fusion protein that inhibited hCG-induced steroid hormone secretion by mouse Leydig tumor cells. Leydig cells were lysed by beta hCG-CH1-3 in the presence of complement. Intravenous injection of beta hCG-CH1-3 in rams was associated with testicular mononuclear leukocyte infiltration, interstitial tissue damage, and a transient depression in circulatory testosterone (levels returned to normal within 2 wk). It appears that targeted cell-killing can be mediated by recombinant proteins composed of the receptor-binding moiety of hormones and truncated effector (Fc) regions of lethal antibodies.