Publications [#326809] of Paul M. Magwene

Papers Published

1. Ehrenreich, IM; Magwene, PM, Genetic Dissection of Heritable Traits in Yeast Using Bulk Segregant Analysis., Cold Spring Harbor protocols, vol. 2017 no. 6 (June, 2017), pp. pdb.prot088989 [doi]
   (last updated on 2024/04/15)

   Abstract:
   Bulk segregant analysis (BSA) is commonly used to determine the genetic basis of complex traits in yeast. This technique involves phenotyping progeny from a cross and then selectively genotyping pooled subsets of offspring with extreme phenotypes. Analysis of these genotype data can identify loci that show skewed allele frequencies in a group of phenotypically extreme individuals and that are likely to contribute to a trait. BSA can be applied to diverse strain crosses, including ones involving nonreference isolates. Further, given the high throughput of next-generation sequencing, it is possible to conduct many BSA experiments in parallel. Here, we present a BSA protocol for the generation of recombinant cross progeny. We then describe general BSA strategies for conducting phenotyping, causal loci detection, and candidate gene identification in a statistically powerful manner.