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Publications [#184715] of Stephanos Venakides
- A. T. Layton1, Y. Toyama, G-Q Yang, G. S. Edwards, D. P. Kiehart, S. Venakides, Drosophila Morphogenesis:Tissue Force Laws and the Modeling of Dorsal Closure,
HFSPHFSP (Human Frontier Science Program) Journal Published online 2009 December 15. doi: 10.2976/1.3266062., vol. 3 no. 6
pp. 441-460, HFSP
(last updated on 2010/12/20)
Dorsal closure, a stage of Drosophila development, is a model system for cell sheet morphogenesis and wound healing. During closure, two flanks of epidermal tissue progressively advance to reduce the area of the eye-shaped opening in the dorsal surface, which contains amnioserosa tissue. To simulate the time evolution of the overall shape of the dorsal opening, we developed a mathematical model, in which contractility and elasticity are manifest in model force-producing elements that satisfy force-velocity relationships similar to muscle. The action of the elements is consistent with the force-producing behavior of actin and myosin in cells. The parameters that characterize the simulated embryos were optimized by reference to experimental observations on wild-type embryos and, to a lesser extent, on embryos whose amnioserosa was removed by laser surgery and on myospheroid mutant embryos. Simulations failed to reproduce the amnioserosa-removal protocol in either the elastic or the contractile limit, indicating that both elastic and contractile dynamics are essential components of the biological force-producing elements. We found it was necessary to actively upregulate forces to recapitulate both the double and single-canthus nick protocols, which did not participate in the optimization of parameters, suggesting the existence of additional key feedback mechanisms.