Department of Mathematics
 Search | Help | Login | pdf version | printable version

Math @ Duke



Publications [#243684] of Anita T. Layton

Papers Published

  1. Hallen, MA; Layton, AT, Expanding the scope of quantitative FRAP analysis, J. Theor. Biol., vol. 2 no. 21 (2010), pp. 295-305 [19836405], [doi]
    (last updated on 2018/11/17)

    In this study, new mathematical models were developed for analysis of fluorescence recovery after photobleaching (FRAP) data to account for features not represented in previous analysis: conical photobleaching geometry, spatial variations in binding of fluorescent molecules, and directed transport of fluorescent molecules. To facilitate computations in conical geometry, a fast computational method for calculation of fluorescence recovery is presented. Two approximations are presented to aid in FRAP analysis when binding varies spatially, one applying to cases of relatively fast diffusion and slow binding and the other to binding of molecules to small cellular structures. Numerical results show that using a model that represents the influential physical processes and that is formulated in the appropriate geometry can substantially improve the accuracy of FRAP calculations.
ph: 919.660.2800
fax: 919.660.2821

Mathematics Department
Duke University, Box 90320
Durham, NC 27708-0320