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Publications [#335962] of Guillermo Sapiro

Papers Published

  1. Bartesaghi, A; Aguerrebere, C; Falconieri, V; Banerjee, S; Earl, LA; Zhu, X; Grigorieff, N; Milne, JLS; Sapiro, G; Wu, X; Subramaniam, S, Atomic Resolution Cryo-EM Structure of β-Galactosidase., Structure (London, England : 1993), vol. 26 no. 6 (June, 2018), pp. 848-856.e3 [doi]
    (last updated on 2019/02/17)

    The advent of direct electron detectors has enabled the routine use of single-particle cryo-electron microscopy (EM) approaches to determine structures of a variety of protein complexes at near-atomic resolution. Here, we report the development of methods to account for local variations in defocus and beam-induced drift, and the implementation of a data-driven dose compensation scheme that significantly improves the extraction of high-resolution information recorded during exposure of the specimen to the electron beam. These advances enable determination of a cryo-EM density map for β-galactosidase bound to the inhibitor phenylethyl β-D-thiogalactopyranoside where the ordered regions are resolved at a level of detail seen in X-ray maps at ∼ 1.5 Å resolution. Using this density map in conjunction with constrained molecular dynamics simulations provides a measure of the local flexibility of the non-covalently bound inhibitor and offers further opportunities for structure-guided inhibitor design.
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