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Publications [#275734] of Amir H. Rezvani

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Papers Published

  1. Rezvani, AH; Denbow, DM; Myers, RD (1987). α-Melanocyte-stimulating hormone (α-MSH) release from perifused rat hypothalamic slices. Brain Research, 413(2), 259-266. [doi]
    (last updated on 2019/02/16)

    Abstract:
    A perifusion sytem was developed to investigate the control of α-melanocyte-stimulating hormone (α-MSH) release from rat brain. Hypothalamic slices were perifused with Krebs-Ringer bicarbonate (KRB) medium supplemented with glucose, bacitracin and bovine serum albumine. Fractions were set apart every 3 min and α-MSH levels were measured by means of a specific and sensitive radioimmunoassay method. Hypothalamic tissue in normal KRB medium released α-MSH at a constant rate corresponding to 0.1% of the total hypothalamic content per 3 min. The basal release was not altered by Ca2+ omission in the medium or addition of the sodium channel blocker tetrodotoxine (TTX). Depolarizing agents such as potassium (50mM) and veratridine (50 μM), which is known to increase Na+ conductance, significantly stimulated α-MSH release in a Ca2+ -dependent manner. When Na+-channels were blocked by TTX (0.5 μM) the stimulatory effect of veratridine was completely abolished whereas the K+-evoked release was unaffected. These findings suggest that: (1) voltage-dependent sodium channels are present on α-MSH hypothalamic neurons; (2) depolarization by K+ induces a marked stimulation of α-MSH release; (3) K+-and veratridine-evoked releases are calcium-dependent. Altogether, these data provide evidence for a neurotransmitter or neuromodulator role for α-MSH in rat hypothalamus.


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