recent faculty publications.
- J. A. Stiber and Z. S. Zhang and J. Burch and J. P. Eu and S. Zhang and G. A. Truskey and M. Seth and N. Yamaguchi and G. Meissner and R. Shah and P. F. Worley and R. S. Williams and P. B. Rosenberg, Mice lacking homer 1 exhibit a skeletal myopathy characterized by abnormal transient receptor potential channel activity,
Molecular And Cellular Biology, vol. 28 no. 8
pp. 2637 -- 2647 .
(last updated on 2011/11/20)
Transient receptor potential (TRIP) channels are nonselective cation channels, several of which are expressed in striated muscle. Because the scaffolding protein Homer 1 has been implicated in TRP channel regulation, we hypothesized that Homer proteins play a significant role in skeletal muscle function. Mice lacking Homer I exhibited a myopathy characterized by decreased muscle fiber cross-sectional area and decreased skeletal muscle force generation. Homer I knockout myotubes displayed increased basal current density and spontaneous cation influx. This spontaneous cation influx in Homer I knockout myotubes was blocked by reexpression of Homer 1b, but not Homer la, and by gene silencing of TRPC1. Moreover, diminished Homer 1 expression in mouse models of Duchenne's muscular dystrophy suggests that loss of Homer I scaffolding of TRP channels may contribute to the increased stretch-activated channel activity observed in mdr myofibers. These findings provide direct evidence that Homer 1 functions as an important scaffold for TRP channels and regulates mechanotransduction in skeletal muscle.