recent faculty publications.
Papers Published
- Burmeister, Jeffrey S. and McKinney, Valerie Z. and Reichert, William M. and Truskey, George A., Role of endothelial cell-substrate contact area and fibronectin-receptor affinity in cell adhesion to HEMA/EMA copolymers,
Journal of Biomedical Materials Research, vol. 47 no. 4
(1999),
pp. 577 - 584 [(SICI)1097-4636(19991215)47:4<577::AID-JBM15>3.0.CO;2-S] .
(last updated on 2007/04/12)Abstract:
In this study, an attempt was made to examine the effect of substrate hydrophobicity on cell-substrate contact area and the affinity between adsorbed fibronectin (Fn) and its receptor. Homo- and copolymer films of hydrophobic ethyl methacrylate (EMA) and hydrophilic hydroxyethyl methacrylate (HEMA) were spin-cast onto glass slides. Bovine aortic endothelial cells (BAEC) were plated for 2 h in serum-free medium onto polymers preadsorbed with Fn. Cells were fixed, labeled, and examined by total internal reflection fluorescence microscopy (TIRFM) to determine the topography of the basal surface as a function of distance from the substrate. The results indicate that differences in the strength of endothelial cell adhesion to polymers are influenced by the conformation of the adsorbed adhesion proteins.Keywords:
Adhesion;Graft copolymers;Hydrophobicity;Adsorption;Microscopic examination;Mathematical models;Affinity chromatography;Conformations;Proteins;Surface properties;