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Papers Published

1. Bhat, V.D. and Truskey, G.A. and Reichert, W.M., Fibronectin and avidin-biotin as a heterogeneous ligand system for enhanced endothelial cell adhesion, Journal of Biomedical Materials Research, vol. 41 no. 3 (1998), pp. 377 - 385 [(SICI)1097-4636(19980905)41:3<377::AID-JBM6>3.0.CO;2-9] .
   (last updated on 2007/04/12)

   Abstract:
   A preadsorbed layer of `heterogeneous' integrin-dependent and -independent protein was used to enhance initial integrin-mediated endothelial cell attachment and spreading. Glass substrates were treated with fibronectin (Fn) and avidin coupled through adsorbed biotinylated bovine serum albumin (b-BSA). The slides then were seeded with biotinylated BAEC. Control `homogeneous' surfaces were slides adsorbed with either Fn or avidin coupled to b-BSA. The cells were incubated for 0.5 h in serum-containing media and exposed to a range of shear stresses in a laminar flow variable-height flow chamber. The critical shear stress to detach 50% of the seeded cells on the heterogeneous ligand surface was significantly greater than for either of the control homogeneous ligand systems (p<0.001). Cellular spreading during the initial period of 0-2 h also was higher (p<0.05) on the heterogeneous ligand-treated surface than on the surface of either of the homogeneous controls. The close contact area of the cell membrane with the substrate 1 h after seeding in serum-containing media was measured using TIRFM. Cells attached onto the heterogeneous ligand-treated surfaces had a significantly (p<0.01) higher area of close contact with the substrate, which is consistent with a greater degree of attachment and spreading. The results indicate that the combination of integrin-dependent and -independent adhesion systems using heterogeneous ligands further enhances initial endothelial cell attachment and spreading.

   Keywords:
   Proteins;Animal cell culture;Adhesion;Cell membranes;Shear stress;Laminar flow;Substrates;Glass;Fluorescence;Microscopic examination;