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Papers Published

1. Olivier, Lauri A. and Reichert, William M. and Truskey, George A., Dynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy, American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, vol. 29 (1995), pp. 565 - 566 .
   (last updated on 2007/04/12)

   Abstract:
   To study the effect of luminal fluid stress on the abluminal cell surface and its adhesion sites, called focal contacts, total internal fluorescence reflectance microscopy is developed for measuring the dynamic response of endothelial cell focal contacts to flow in vitro. This method is used to illuminate the lower cell membrane so that focal contact movement can be followed over time. Based on the results, it is indicated that abluminal cell contour modulation occurs at a moderate level under static and low shear conditions, increasing substantially at higher shear rates. Moreover, at early flow time, the cell contour is moving away from the surface and focal contact sites are decreasing in response to the fluid stress.

   Keywords:
   Cells;Microscopic examination;Fluorescence;Fluids;Calculations;Correlation methods;Shear stress;Dyes;Pressure;Fluid mechanics;Image processing;Mathematical transformations;