Publications [#113148] of Harold P. Erickson
- CY Chung, JE Murphy-Ullrich, HP Erickson, Mitogenesis, cell migration, and loss of focal adhesions induced by tenascin-C interacting with its cell surface receptor, annexin II.,
Molecular biology of the cell, UNITED STATES, vol. 7 no. 6
pp. 883-92, ISSN 1059-1524
(last updated on 2009/02/12)
In a previous study we demonstrated that the alternatively spliced region of tenascin-C, TNfnA-D, bound with high affinity to a cell surface receptor, annexin II. In the present study we demonstrate three changes in cellular activity that are produced by adding intact tenascin-C or TNfnA-D to cells, and we show that all three activities are blocked by antibodies against annexin II. 1) TNfnA-D added to confluent endothelial cells induced loss of focal adhesions. 2) TNfnA-D produced a mitogenic response of confluent, growth-arrested endothelial cells in 1% serum. TNfnA-D stimulated mitogenesis only when it was added to cells before or during exposure to other mitogens, such as basic fibroblast growth factor or serum. Thus the effect of TNfnA-D seems to be to facilitate the subsequent response to growth factors. 3) TNfnA-D enhanced cell migration in a cell culture wound assay. Antibodies to annexin II blocked all three cellular responses to TNfnA-D. These data show that annexin II receptors on endothelial cells mediate several cell regulatory functions attributed to tenascin-C, potentially through modulation of intracellular signalling pathways.
Animals • Annexin A2 • Antibodies • Cattle • Cell Adhesion • Cell Division • Cell Line • Cell Movement • Drug Interactions • Endothelium, Vascular • Growth Substances • Mitogens • Receptors, Cell Surface • Tenascin • antagonists & inhibitors • biosynthesis* • cytology • drug effects* • immunology • pharmacology • pharmacology* • physiology*