Publications [#62087] of Kam W. Leong

Papers Published

1. Lim, W.S. and Chua, K.N. and Wang, X.L. and Zhang, P.C. and Lu, H.F. and Leong, K.W. and Mao, H.Q., Transfection of rat primary hepatocytes using a novel polyphosphoramidate gene carrier, Transactions - 7th World Biomaterials Congress (2004), pp. 1813 -, Sydney, Australia
   (last updated on 2007/04/13)

   Abstract:
   The transfectability of the hepatocyte spheroids in comparison with hepatocyte monolayer was investigated using a novel non-viral polycationic gene carrier, polyphosphoramidate containing diethylamine side chains (PPA-DEA). The optimal window for transfection after cell seeding and culture was also determined. Using ELISA and HPLC techniques, hepatocyte-specific functions such as albumin secretion and P450 activity were measured. PPA-DEA was found to be a viable non-viral gene carrier since it produces reasonable transgene expression and has no adverse effects on the hapatocyte-specific functions.

   Keywords:
   Cells;Proteins;Monolayers;Collagen;Tissue;Polystyrenes;Synthesis (chemical);DNA;High performance liquid chromatography;Polyethylene terephthalates;