Publications [#161998] of Ashutosh Chilkoti

Papers Published

  1. A. Valiaev and D. W. Lim and S. Schmidler and R. L. Clark and A. Chilkoti and S. Zauscher, Hydration and conformational mechanics of single, end-tethered elastin-like polypeptides, Journal Of The American Chemical Society, vol. 130 no. 33 (August, 2008), pp. 10939 -- 10946, ISSN 0002-7863
    (last updated on 2009/09/02)

    Abstract:
    We investigated the effect of temperature, ionic strength, solvent polarity, and type of guest residue on the force-extension behavior of single, end-tethered elastin-like polypeptides (ELPs), using single molecule force spectroscopy (SMFS). ELPs are stimulus-responsive polypeptides that contain repeats of the five amino acids Val-Pro-Gly-Xaa-Gly (VPGXG), where Xaa is a guest residue that can be any amino acid with the exception of proline. We fitted the force-extension data with a freely jointed chain (FJC) model which allowed us to resolve small differences in the effective Kuhn segment length distributions that largely arise from differences in the hydrophobic hydration behavior of ELP. Our results agree qualitatively with predictions from recent molecular dynamics simulations and demonstrate that hydrophobic hydration modulates the molecular elasticity for ELPs. Furthermore, our results show that SMFS, when combined with our approach for data analysis, can be used to study the subtleties of polypeptide-water interactions and thus provides a basis for the study of hydrophobic hydration in intrinsically unstructured biomacromolecules.