Publications [#260892] of Ashutosh Chilkoti

Papers Published

1. Chow, DC; Chilkoti, A, Surface-initiated enzymatic polymerization of DNA., Langmuir : the ACS journal of surfaces and colloids, vol. 23 no. 23 (November, 2007), pp. 11712-11717, ISSN 0743-7463 [17929953], [doi]
   (last updated on 2024/04/24)

   Abstract:
   We describe a technique to synthesize DNA homopolymers on a surface using surface-initiated enzymatic polymerization (SIEP) with terminal deoxynucleotidyl transferase (TdTase), an enzyme that repetitively adds mononucleotides to the 3'-end of oligonucleotides. The thickness of the synthesized DNA layer was found to depend on the deoxymononucleotide monomer, in the order of dATP > dTTP > dGTP approximately dCTP. In addition, the composition and the surface density of oligonucleotide initiators were also important in controlling the extent of DNA polymerization. The extension of single-stranded DNA chains by SIEP was further verified by their binding to antibodies specific to oligonucleotides. TdTase-mediated SIEP can also be used to grow spatially defined three-dimensional DNA structures by soft lithography and is a new tool for bioinspired fabrication at the micro- and nanoscale.