Publications [#61141] of Ashutosh Chilkoti

Papers Published

1. Dreher, M.R. and Elas, M. and Ichikawa, K. and Barth, E.D. and Chilkoti, A. and Rosen, G.M. and Halpern, H.J. and Dewhirst, M., Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry, Med. Phys. (USA), vol. 31 no. 10 (2004), pp. 2755 - 62 [1.1782677]
   (last updated on 2007/04/12)

   Abstract:
   Hyperthermia, as an adjuvant with radiation and chemotherapy, has shown promise in the treatment of cancer. The relevant biological effects of a hyperthermia treatment are both time and temperature-dependent, creating a need for accurate thermometry. We present a novel noninvasive thermometry modality that combines a temperature responsive biopolymer, the elastin-like polypeptide (ELP), and nitroxide to produce an ELP-nitroxide conjugate. When examined with electron paramagnetic resonance (EPR) spectroscopy, the ELP-nitroxide conjugate has temperature-dependent spectral line widths whose predictive accuracy is ~0.3°C (80 μM). We believe that the temperature-dependent changes observed in the EPR spectrum are due to the combined effect of temperature, viscosity and effective radius on the rotational correlation time of the ELP-nitroxide conjugate

   Keywords:
   biochemistry;cancer;EPR line breadth;EPR spectroscopy;hyperthermia;proteins;tumours;