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| Publications [#168898] of Mark W. Dewhirst
Papers Published
- F Siddiqui, CY Li, X Zhang, SM Larue, MW Dewhirst, RL Ullrich, PR Avery, Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy.,
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, England, vol. 22 no. 2
(March, 2006),
pp. 117-34, ISSN 0265-6736
(last updated on 2009/12/31)
Abstract:
Interleukin-12 (IL-12) is a pro-inflammatory cytokine that has shown great promise as a therapeutic agent in experimental models of infectious disease and cancer. However, it is also a highly toxic molecule and for that reason has not been accepted readily into the clinic. A replication-deficient adenoviral vector was designed to deliver the feline interleukin-12 gene into tumour cells. The interleukin-12 gene has been placed under control of a heat inducible promoter, human heat shock promoter 70b, with the intent of spatially and temporally controlling the expression of IL-12, thus limiting its toxicity. In vitro, the transfection efficiency of the adenoviral vector, the effect of multiplicity of infection and the production of biologically active feline IL-12 were studied in the infected cells in response to a range of temperatures. This adenoviral vector will be a useful tool to examine the effects of intra-tumoural IL-12 delivery in a spontaneously occurring feline soft tissue sarcoma model.
Keywords:
Adenoviridae • Animals • Cats • Cell Line • DNA • DNA, Viral • Gene Expression Regulation • Gene Therapy • Genetic Vectors • HSP70 Heat-Shock Proteins • Humans • Hyperthermia, Induced • Interferon-gamma • Interleukin-12 • Leukocytes, Mononuclear • Promoter Regions, Genetic • RNA, Messenger • Sarcoma • Temperature • Transfection • Transgenes • analysis • genetics • genetics* • metabolism • methods* • physiology • therapy
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