Publications [#169007] of Mark W. Dewhirst
- F Li, P Sonveaux, ZN Rabbani, S Liu, B Yan, Q Huang, Z Vujaskovic, MW Dewhirst, CY Li, Regulation of HIF-1alpha stability through S-nitrosylation.,
Molecular cell, vol. 26 no. 1
pp. 63-74, ISSN 1097-2765 [doi]
(last updated on 2013/05/16)
Hypoxia-inducible factor 1 (HIF-1) is a master transcriptional factor. Under normal oxygen tension, HIF-1 activity is usually suppressed due to the rapid, oxygen-dependent degradation of one of its two subunits, HIF-1alpha. Here we report that normoxic HIF-1 activity can be upregulated through NO-mediated S-nitrosylation and stabilization of HIF-1alpha. In murine tumors, exposure to ionizing radiation stimulated the generation of NO in tumor-associated macrophages. As a result, the HIF-1alpha protein is S-nitrosylated at Cys533 (through "biotin switch" assay) in the oxygen-dependent degradation domain, which prevents its destruction. Importantly, this mechanism appears to be independent of the prolylhydroxylase-based pathway that is involved in oxygen-dependent regulation of HIF-1alpha. Selective disruption of this S-nitrosylation significantly attenuated both radiation-induced and macrophage-induced activation of HIF-1alpha. This interaction between NO and HIF-1 sheds new light on their involvement in tumor response to treatment as well as mammalian inflammation process in general.
Amino Acid Sequence • Animals • Cell Line, Tumor • Cells, Cultured • Cysteine • Gene Expression Regulation, Neoplastic* • Hypoxia-Inducible Factor 1, alpha Subunit • Macrophages • Mice • Models, Biological • Molecular Sequence Data • Mutation • Neoplasms • Nitric Oxide • Nitric Oxide Synthase Type II • Sequence Homology, Amino Acid • Transfection • chemistry • enzymology • genetics • metabolism • metabolism* • radiation effects • radiotherapy