Publications [#63278] of Mark W. Dewhirst

Papers Published

1. Snyder, S.A. and Lanzen, J.L. and Braun, R.D. and Rosner, G. and Secomb, T.W. and Biaglow, J. and Brizel, D.M. and Dewhirst, M.W., Simultaneous administration of glucose and hyperoxic gas achieves greater improvement in tumor oxygenation than hyperoxic gas alone, International Journal of Radiation Oncology Biology Physics, vol. 51 no. 2 (2001), pp. 494 - 506 [S0360-3016(01)01654-6]
   (last updated on 2007/04/14)

   Abstract:
   Purpose: To test the feasibility of hyperglycemic reduction of oxygen consumption combined with oxygen breathing (O₂), to improve tumor oxygenation. Methods and Materials: Fischer-344 rats bearing 1 cm R3230Ac flank tumors were anesthetized with Nembutal. Mean arterial pressure, heart rate, tumor blood flow ([TBF], laser Doppler flowmetry), pH, and pO₂ were measured before, during, and after glucose (1 or 4 g/kg) and/or O₂. Results: Mean arterial pressure and heart rate were unaffected by treatment. Glucose at 1 g/kg yielded maximum blood glucose of 400 mg/dL, no change in TBF, reduced tumor pH (0.17 unit), and 3 mm Hg pO₂ rise. Glucose at 4 g/kg yielded maximum blood glucose of 900 mg/dL, pH drop of 0.6 unit, no pO₂ change, and reduced TBF (31%). Oxygen tension increased by 5 mm Hg with O₂. Glucose (1 g/Kg) + O₂ yielded the largest change in pO₂ (27 mm Hg); this is highly significant relative to baseline or either treatment alone. The effect was positively correlated with baseline pO₂, but 6 of 7 experiments with baseline pO₂ less than or equal 10 mm Hg rose above 10 mm Hg after combined treatment. Conclusion: We demonstrated the feasibility of combining hyperglycemia with O₂ to improve tumor oxygenation. However, some cell lines are not susceptible to the Crabtree effect, and the magnitude is dependent on baseline pO₂. Additional or alternative manipulations may be necessary to achieve more uniform improvement in pO₂. Copyright © 2001 Elsevier Science Inc.

   Keywords:
   Tumors;Oxygen;Blood;Glucose;Patient treatment;