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Pratt School of Engineering
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Publications [#119315] of Bernard M Fischer

Papers Published

  1. KB Adler, BM Fischer, H Li, NH Choe, DT Wright, Hypersecretion of mucin in response to inflammatory mediators by guinea pig tracheal epithelial cells in vitro is blocked by inhibition of nitric oxide synthase., American journal of respiratory cell and molecular biology, vol. 13 no. 5 (November, 1995), pp. 526-30, ISSN 1044-1549 [doi]
    (last updated on 2013/05/16)

    Primary cultures of guinea pig tracheal epithelial cells in air/liquid interface were exposed to one of four agents associated with airway inflammation: the peptide histamine (100 microM), the lipid mediator platelet-activating factor (1 microM), the cytokine tumor necrosis factor-alpha (15 ng/ml; specific activity 2.86 x 10(7) U/mg), or enzymatically generated reactive oxygen species (purine [500 microM]+xanthine oxidase [20 mU/ml]). Effects of each of these substances on release of mucin by guinea pig tracheal epithelial (GPTE) cells were measured using a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA). Each secretagogue significantly enhanced release of mucin, but the stimulatory effect of each was inhibited by pre-(+)co-incubation of the cells with the competitive inhibitor of nitric oxide synthase, NG-monomethyl-L-arginine (L-NMA), but not by NG-monomethyl-D-arginine (D-NMA), the inactive stereoisomer that does not inhibit nitric oxide synthase. Neither L-NMA nor D-NMA affected mucin secretion by themselves. The results suggest that each of these inflammation-associated mediators provokes airway epithelial mucin secretion via a mechanism involving intracellular production of nitric oxide (NO) as a critical signaling molecule.

    Animals • Arginine • Cells, Cultured • Enzyme Inhibitors • Guinea Pigs • Histamine • Inflammation Mediators • Mucins • Nitric Oxide Synthase • Platelet Activating Factor • Secretory Rate • Superoxides • Trachea • Tumor Necrosis Factor-alpha • analogs & derivatives • antagonists & inhibitors* • drug effects • metabolism • omega-N-Methylarginine • pharmacology • pharmacology* • secretion*

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