Publications [#132819] of G. Allan Johnson
- BR Smith, E Linney, DS Huff, GA Johnson, Magnetic resonance microscopy of embryos.,
Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society, UNITED STATES, vol. 20 no. 6
pp. 483-90, ISSN 0895-6111
(last updated on 2005/08/03)
We demonstrate that magnetic resonance (MR) microscopy provides a mechanism to investigate normal and abnormal developmental anatomy in a non-destructive and distortion-free manner. Techniques for the fixation, embedding, perfusion and image acquisition of embryos between 3 and 30 mm crown rump length are described. We describe the perfusion of a contrast agent to enhance images of the developing embryonic vasculature. Data are acquired as three-dimensional isotropic arrays which permit images to be reformatted retrospectively in any plane. The data are available for archiving, distributing and for post-acquisition manipulations. MR microscopy is a fast technique for producing three-dimensional reconstructions and is free from registration and sectioning artifacts.
Anatomy, Artistic • Animals • Contrast Media • Databases, Factual • Embryo • Embryonic and Fetal Development* • Humans • Image Processing, Computer-Assisted • Magnetic Resonance Imaging* • Medical Illustration • Mice • Microscopy • anatomy & histology • anatomy & histology* • embryology