Publications [#132860] of G. Allan Johnson
- X Zhou, RR Maronpot, LW Hedlund, GP Cofer, GA Johnson, Detection of bromobenzene-induced hepatocellular necrosis using magnetic resonance microscopy.,
Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, UNITED STATES, vol. 34 no. 6
pp. 853-7, ISSN 0740-3194
(last updated on 2005/08/03)
The authors used magnetic resonance (MR) microscopy to assess hepatic tissue damage induced by bromobenzene both in living rats and in fixed rat liver tissues. Experiments were conducted at 7 Tesla on three groups of Fisher rats treated with bromobenzene at a single dose of 68, 135, and 269 mg/kg, respectively. Optical microscopy of hematoxylin and eosin stained sections showed liver damage only at the highest dose, whereas with MR microscopy, tissue alterations were detected at all three doses both in vivo and ex vivo. The contrast mechanism of the superior sensitivity of MR microscopy is believed to be related to the changes in local diffusion coefficients that accompany cellular degeneration and death, although other contrast mechanisms may also be involved. The superior sensitivity of MR microscopy, as demonstrated in this study, has many implications for potential use of MR techniques to perform in vivo histology.
Animals • Bromobenzenes • Dose-Response Relationship, Drug • Female • Image Processing, Computer-Assisted • Liver • Magnetic Resonance Spectroscopy • Necrosis • Rats • Rats, Inbred F344 • Sensitivity and Specificity • diagnostic use* • drug effects* • methods • pathology • toxicity*