Publications [#157115] of G. Allan Johnson
- S Shofer, C Badea, S Auerbach, DA Schwartz, GA Johnson, A micro-computed tomography-based method for the measurement of pulmonary compliance in healthy and bleomycin-exposed mice.,
Experimental lung research, United States, vol. 33 no. 3-4
pp. 169-83, ISSN 0190-2148
(last updated on 2009/03/13)
Micro-computed tomography (microCT) is being increasingly used to examine small animal models of pulmonary injury. The authors have developed a microCT technique suitable for the determination of pulmonary compliance in injured mice. Lung volumes in normal mice were radiographically determined at end-inspiration and end-expiration and pulmonary compliance was calculated at 2 time points 2 weeks apart, whereas a second group of mice were given bleomycin and imaged 3 weeks following drug administration. Compliance measurements were validated using a commercially available ventilator system. MicroCT pulmonary compliance measurements are suitable for longitudinal measurements, and correlate with physiologic measurements of pulmonary compliance.
Animals • Bleomycin • Disease Models, Animal • Female • Image Processing, Computer-Assisted • Lung • Lung Compliance* • Male • Mice • Mice, Inbred C57BL • Pulmonary Fibrosis • Reproducibility of Results • Time Factors • Tomography, X-Ray Computed • chemically induced • methods* • pathology • physiopathology • radiography*