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Publications [#161595] of G. Allan Johnson

Papers Published

  1. DG Johnston, GA Johnson, KG Alberti, GH Millward-Sadler, J Mitchell, R Wright, Hepatic regeneration and metabolism after partial hepatectomy in normal rats: effects of insulin therapy., European journal of clinical investigation, ENGLAND, vol. 16 no. 5 (October, 1986), pp. 376-83, ISSN 0014-2972
    (last updated on 2009/08/26)

    Abstract:
    The effect of insulin therapy on liver regeneration has been studied in normal fed rats 12, 24 and 48 h after partial hepatectomy. Dry weight of regenerating liver increased between 12 and 48 h after partial hepatectomy and was unaffected by insulin therapy. [6-3H] Thymidine uptake peaked at 24-h (24.7 +/- 2.4% of total liver cells) and insulin treatment had no additional effect. At 12-h after partial hepatectomy, hepatic [ATP] was decreased 15%, while [ADP] and [AMP] were increased 47% and 83% respectively compared with sham-operated animals. Partial hepatectomy also caused an increase in hepatic [triglyceride], a decrease in hepatic [glycogen] and an increase in the levels of glucose and several glycolytic intermediates. The hepatic redox ratios, [lactate]:[pyruvate] and [3-hydroxybutyrate]:[acetoacetate], were elevated. Insulin therapy had only minor effects on hepatic adenine nucleotide levels, intermediary metabolite concentrations or intrahepatic redox ratios after partial hepatectomy. These findings suggest a decreased hepatic intracellular energy state in regenerating liver; insulin therapy in normal rats does not influence this metabolic change nor the regenerative response.

    Keywords:
    Adenine Nucleotides • Animals • DNA • Glucose • Glycogen • Hepatectomy* • Insulin • Liver • Liver Regeneration • Male • Organ Size • Rats • Rats, Inbred Strains • Thymidine • Water • analysis • biosynthesis • cytology • drug effects* • metabolism • metabolism* • therapeutic use*


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