Publications [#174153] of G. Allan Johnson

Papers Published

1. RC Ackerman, GA Johnson, EA Van Kirk, AL Asirvatham, WJ Murdoch, Induction of apoptotic or lytic death in an ovarian adenocarcinoma cell line by antibodies generated against a synthetic N-terminal extracellular domain gonadotropin-releasing hormone receptor peptide., Cancer letters, vol. 81 no. 2 (June, 1994), pp. 177-84, ISSN 0304-3835
   (last updated on 2010/04/30)

   Abstract:
   A polyclonal antiserum was generated in ovariectomized sheep against a synthetic peptide corresponding to amino acids 5-17 of the deduced mouse pituitary gonadotropin-releasing hormone (GnRH) receptor. Antipeptide antibodies did not bind native cells, but did react strongly with a human ovarian cancer cell line (OVCAR-3) reportedly sensitive to GnRH. Growth of cultured OVCAR-3 cells was specifically suppressed by antipeptide serum. This was attributed in part to programmed death (chromatin condensation and DNA fragmentation) of cells by antibody-induced apoptosis. Antibodies also exhibited a cytolytic effect (lactate dehydrogenase release) toward OVCAR-3 cells in the presence of the complement. Endometria of passively immunized mice lacked development; thus, antipeptide antibodies evidently recognize Mullerian duct derivatives. Experiments are in progress to determine whether the putative antigen is a variant of the pituitary GnRH receptor or a largely dissimilar protein. Effector-functional antibodies could be useful in the management of ovarian or uterine neoplasia.

   Keywords:
   Adenocarcinoma • Amino Acid Sequence • Animals • Apoptosis* • Cytotoxicity, Immunologic • Female • Humans • Immune Sera • Mice • Molecular Sequence Data • Ovarian Neoplasms • Peptide Fragments • Receptors, LHRH • Sheep • Tumor Cells, Cultured • immunology* • pathology*