Fitzpatrick Institute for Photonics Fitzpatrick Institute for Photonics
Pratt School of Engineering
Duke University

 HOME > pratt > FIP    Search Help Login pdf version printable version 

Publications [#174235] of G. Allan Johnson

Papers Published

  1. GA Johnson, TE Spencer, TR Hansen, KJ Austin, RC Burghardt, FW Bazer, Expression of the interferon tau inducible ubiquitin cross-reactive protein in the ovine uterus., Biology of reproduction, vol. 61 no. 1 (July, 1999), pp. 312-8, ISSN 0006-3363
    (last updated on 2010/04/30)

    Abstract:
    Ubiquitin cross-reactive protein (UCRP) is a 17-kDa protein that shows cross-reactivity with ubiquitin antisera and retains the carboxyl-terminal Leu-Arg-Gly-Gly amino acid sequence of ubiquitin that ligates to, and directs degradation of, cytosolic proteins. It has been reported that bovine endometrial UCRP is synthesized and secreted in response to conceptus-derived interferon-tau (IFNtau). In the present studies, UCRP mRNA and protein were detected in ovine endometrium. Ovine UCRP mRNA was detectable on Day 13, peaked at Day 15, and remained high through Day 19 of pregnancy. The UCRP mRNA was localized to the luminal epithelium (LE), stromal cells (ST) immediately beneath the LE, and shallow glandular epithelium (GE) on Day 13, but it extended to the deep GE, deep ST, and myometrium of uterine tissues by Day 15 of pregnancy. Western blotting revealed induction of UCRP in the endometrial extracts from pregnant, but not cyclic, ewes. Ovine UCRP was also detected in uterine flushings from Days 15 and 17 of pregnancy and immunoprecipitated from Day 17 pregnant endometrial explant-conditioned medium. Treatment of immortalized ovine LE cells with recombinant ovine (ro) IFNtau induced cytosolic expression of UCRP, and intrauterine injection of roIFNtau into ovariectomized cyclic ewes induced endometrial expression of UCRP mRNA. These results are the first to describe temporal and spatial alterations in the cellular localization of UCRP in the ruminant uterus. Collectively, UCRP is synthesized and secreted by the ovine endometrium in response to IFNtau during early pregnancy. Because UCRP is present in the uterus and uterine flushings, it may regulate endometrial proteins associated with establishment and maintenance of early pregnancy in ruminants.

    Keywords:
    Animals • Blotting, Northern • Blotting, Western • Cattle • Endometrium • Epithelium • Female • Gene Expression Regulation* • Gestational Age • In Situ Hybridization • Interferon Type I • Ovariectomy • Pregnancy • Pregnancy Proteins • RNA, Messenger • Recombinant Proteins • Sheep* • Stromal Cells • Ubiquitins • Uterus • analogs & derivatives* • analysis • chemistry • genetics • metabolism* • pharmacology • pharmacology*


Duke University * Pratt * Reload * Login