Publications [#60894] of George A. Truskey

Papers Published

1. Burmeister, Jeffrey S. and McKinney, Valerie Z. and Reichert, William M. and Truskey, George A., Role of endothelial cell-substrate contact area and fibronectin-receptor affinity in cell adhesion to HEMA/EMA copolymers, Journal of Biomedical Materials Research, vol. 47 no. 4 (1999), pp. 577 - 584 [(SICI)1097-4636(19991215)47:4<577::AID-JBM15>3.0.CO;2-S]
   (last updated on 2007/04/12)

   Abstract:
   In this study, an attempt was made to examine the effect of substrate hydrophobicity on cell-substrate contact area and the affinity between adsorbed fibronectin (Fn) and its receptor. Homo- and copolymer films of hydrophobic ethyl methacrylate (EMA) and hydrophilic hydroxyethyl methacrylate (HEMA) were spin-cast onto glass slides. Bovine aortic endothelial cells (BAEC) were plated for 2 h in serum-free medium onto polymers preadsorbed with Fn. Cells were fixed, labeled, and examined by total internal reflection fluorescence microscopy (TIRFM) to determine the topography of the basal surface as a function of distance from the substrate. The results indicate that differences in the strength of endothelial cell adhesion to polymers are influenced by the conformation of the adsorbed adhesion proteins.

   Keywords:
   Adhesion;Graft copolymers;Hydrophobicity;Adsorption;Microscopic examination;Mathematical models;Affinity chromatography;Conformations;Proteins;Surface properties;