Publications [#113155] of Harold P. Erickson

Papers Published

1. J Takagi, DP DeBottis, HP Erickson, TA Springer, The role of the specificity-determining loop of the integrin beta subunit I-like domain in autonomous expression, association with the alpha subunit, and ligand binding., Biochemistry, United States, vol. 41 no. 13 (April, 2002), pp. 4339-47, ISSN 0006-2960
   (last updated on 2009/02/12)

   Abstract:
   Integrin beta subunits contain a highly conserved I-like domain that is known to be important for ligand binding. Unlike integrin I domains, the I-like domain requires integrin alpha and beta subunit association for optimal folding. Pactolus is a novel gene product that is highly homologous to integrin beta subunits but lacks associating alpha subunits [Chen, Y., Garrison, S., Weis, J. J., and Weis, J. H. (1998) J. Biol. Chem. 273, 8711-8718] and a approximately 30 amino acid segment corresponding to the specificity-determining loop (SDL) in the I-like domain. We find that the SDL is responsible for the defects in integrin beta subunit expression and folding in the absence of alpha subunits. When transfected in the absence of alpha subunits into cells, extracellular domains of mutant beta subunits lacking SDL, but not wild-type beta subunits, were well secreted and contained immunoreactive I-like domains. The purified recombinant soluble beta1 subunit with the SDL deletion showed an elongated shape in electron microscopy, consistent with its structure in alphabeta complexes. The SDL segment is not required for formation of alpha5beta1, alpha4beta1, alphaVbeta3, and alpha6beta4 heterodimers, but is essential for fomation of alpha6beta1, alphaVbeta1, and alphaLbeta2 heterodimers, suggesting that usage of subunit interface residues is variable among integrins. The beta1 SDL is required for ligand binding and for the formation of the epitope for the alpha5 monoclonal antibody 16 that maps to loop segments connecting blades 2 and 3 of beta-propeller domain of alpha5, but is not essential for nearby beta-propeller epitopes.

   Keywords:
   Amino Acid Sequence • Animals • Antibodies, Monoclonal • Antigens, CD18 • Antigens, CD29 • Cell Adhesion • Cell Line • Dimerization • Electrophoresis, Polyacrylamide Gel • Epitopes • Fibronectins • Gene Deletion • Humans • Ligands • Mice • Models, Molecular • Molecular Sequence Data • Mutation • Precipitin Tests • Protein Binding • Protein Folding • Protein Structure, Secondary • Protein Structure, Tertiary • Recombinant Proteins • Sequence Homology, Amino Acid • Transfection • chemistry • chemistry*