| Publications [#193305] of Harold P. Erickson
Papers Published
- M Osawa, HP Erickson, Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings.,
Methods in enzymology, vol. 464
(2009),
pp. 3-17, ISSN 1557-7988 [doi]
(last updated on 2013/05/16)
Abstract: We have developed a system for producing tubular multilamellar liposomes that incorporate the protein FtsZ on the inside. We start with a mixture of spherical multilamellar liposomes with FtsZ initially on the outside. Shearing forces generated by applying a coverslip most likely distort some of the spherical liposomes into a tubular shape, and causes some to leak and incorporate FtsZ inside. We describe protocols for liposome preparation, and for preparing membrane-targeted FtsZ that can assemble contractile Z rings inside the tubular liposomes. We also describe the characterization of the multilamellar liposomes in terms of the permeability or leakiness for a small fluorescent dye and larger protein molecules. These liposomes may be useful for reconstitution of other biological systems.
Keywords: Bacterial Proteins • Cytoskeletal Proteins • Liposomes • Membrane Proteins • Molecular Biology • Permeability • chemistry • metabolism* • methods*
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