Publications [#62347] of Nimmi Ramanujam

Papers Published

1. Durkin, A.J. and Jaikumar, S. and Ramanujam, N. and Richards-Kortum, R., Relation between fluorescence spectra of dilute and turbid samples, Appl. Opt. (USA), vol. 33 no. 3 (1994), pp. 414 - 23
   (last updated on 2007/04/13)

   Abstract:
   We present a method to extend rank-annihilation-factor analysis (RAFA) for the analysis of fluorescence from homogeneous turbid samples. The method is based on a fundamental relationship between the fluorescence of a dilute solution and that of a turbid solution. We have derived this relationship, known as the transfer function, for turbid materials using the two-flux Kubelka-Munktheory theory. The method is tested with spectroscopic data from optically thin and turbid samples of the media of a human aorta. At 450-nm excitation, agreement between the measured and predicted dilute-solution fluorescence spectra is within 5% at all emission wavelengths; at 340-nm excitation, agreement is within 20% at all wavelengths, with some residual Soret-band absorption. The simulations presented indicate that the transfer function is markedly more sensitive to absorption than to scattering properties

   Keywords:
   biological techniques and instruments;fluorescence;light scattering;visible spectra of organic molecules and substances;