Publications [#283668] of Qing H. Liu

Papers Published

1. Huang, HX; Li, Y; Liu, QS; Liu, QH; Chen, YD; Wan, L; Zhuang, H; Zhao, KW, Effects of Chinese herbs of tonifying kidney on bone mineral density and contents of insulin-like growth factor-1 and tumor necrosis factor-alpha in osteoporotic rats, Journal of Clinical Rehabilitative Tissue Engineering Research, vol. 12 no. 37 (2008), pp. 7219-7222, ISSN 1673-8225
   (last updated on 2023/08/08)

   Abstract:
   Background: Studies have shown that insulin growth factor and tumor necrosis factor α (TNF- α) can regulate osteoblast and osteoclast. Objective: To explore the effect of Chinese medicine prescription of tonifying kidney on bone mineral density, insulin-like growth factor-1 (IGF-1) and TNF- α in osteoporotic rats. Design, time and setting: Randomized controlled animal trial was performed at Animal Center and Orthopedic Hospital of Guangzhou University of Chinese Medicine from September 2006 to March 2007. Materials: Sixty-eight SPF 6-month-old healthy female SD rats weighing 260-280 g were randomly divided into normal group (n=22) and model group (n=46). Three months after surgery, 10 rats from each group were selected to confirm modeling. The other rats in ovariectomized group were randomly divided into 3 groups (n=12): ovariectomized group (OVX), estrogen group, and traditional Chinese herb group. The other 12 rats in the normal group served as control group. The traditional Chinese herb consisted of Yinyanghuo, Buguzhi, Danshen, Huangqi and other six kinds of herbs containing crude drug of 1 430 g/L, prepared by Manufacturing Laboratory of Orthopaedic Hospital of Guangzhou University of Chinese Medicine. Methods: The rats of traditional Chinese herb group were intragastrically administrated with herbs (4.8 g/kg), once a day. The rats of estrogen group were intragastrically infused nilestriol (1 mg/ kg), once a week. The others were given distilled water (10 mL/kg), once a day. The administration lasted for 3 months. Main outcome measures: Total body, lumbar vertebral and hip bone mineral density (BMD) was measured by dual energy X-ray absorptiometry, and the content of IGF-1, estrogen and TNF- α in blood serum were detected with radioimmunoassay. Results: Sixty-four rats were included in the final analysis. The BMD of OVX group was significantly decreased compared with the control group (P < 0.05). After treatment, the BMD of the total body and lumbar vertebrae in the rats of Chinese herb and estrogen groups were significant higher than OVX group (P < 0.05), but there were no significant differences between Chinese herb and estrogen groups (P > 0.05). Compared with OVX group, Chinese herbs markedly reduced TNF- α levels, and increased IGF-1 and estrogen content (P < 0.05), but there were no significant differences compared with estrogen group. Estrogen content in estrogen group was significantly lower than the other groups. Conclusion: Chinese medicine prescription of tonifying kidney plays estrogen-like role in treating osteoporosis, but has different influence on IGF-1 from estrogen.