- Frey, Wolfgang and Meyer, Dan E. and Chilkoti, Ashutosh, Dynamic addressing of a surface pattern by a stimuli-responsive fusion protein,
Advanced Materials, vol. 15 no. 3
pp. 248 - 251 [adma.200390058] .
(last updated on 2007/04/12)
An overview is given of a dynamic protein patterning methodology, thermodynamically reversible addressing of proteins (TRAP), in which a recombinant, stimuli responsive polypeptide is used to dynamically address a fusion protein onto a micropatterned surface template by modulating the interaction between a chemically patterned surface and the stimuli-responsive polypeptide. Immobilization of the fusion protein in spatially defined regions of the micropatterned surface is triggered either by an increase in temperature or ionic strength and the immobilized fusion partner is oriented towards solution and is recognized by an antibody to the fusion protein. TRAP is reversible, because the protein-antibody complex can be resolubilized from the surface by reversing the environmental trigger.
Surfaces;Polypeptides;Ionic strength;Synthesis (chemical);Oligomers;Phase transitions;Temperature;pH;Light scattering;Sodium chloride;Atomic force microscopy;