- Hoffman, Allan S. and Stayton, Patrick S. and Bulmus, Volga and Chen, Guohua and Chen, Jingping and Cheung, Chuck and Chilkoti, Ashutosh and Ding, Zhongli and Dong, Liangchang and Fong, Robin and Lackey, Chantal A. and Long, Cynthia J. and Miura, Morikazu and Morris, John E. and Murthy, Niren and et al., Really smart bioconjugates of smart polymers and receptor proteins,
Journal of Biomedical Materials Research, vol. 52 no. 4
pp. 577 - 586 [1097-4636(20001215)52:4<577::AID-JBM1>3.0.CO;2-5] .
(last updated on 2007/04/12)
Over the past 18 years we have been deeply involved with the synthesis and applications of stimuliresponsive polymer systems, especially polymer-biomolecule conjugates. This article summarizes our work with one of these conjugate systems, specifically polymer-protein conjugates. We include conjugates prepared by random polymer conjugation to lysine amino groups, and also those prepared by site-specific conjugation of the polymer to specific amino acid sites that are genetically engineered into the known amino acid sequence of the protein. We describe the preparation and properties of thermally sensitive random conjugates to enzymes and several affinity recognition proteins. We have also prepared site-specific conjugates to streptavidin with temperature-sensitive polymers, pH-sensitive polymers, and light-sensitive polymers. The preparation of these conjugates and their many fascinating applications are reviewed in this article.
Proteins;Organic polymers;Intelligent materials;Thermal effects;pH effects;Bioassay;