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Papers Published

  1. Zhang, Zhanping and Ma, Hongwei and Hausner, Douglas B. and Chilkoti, Ashutosh and Beebe Jr., Thomas P., Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption, Biomacromolecules, vol. 6 no. 6 (2005), pp. 3388 - 3396 [bm050446d] .
    (last updated on 2007/04/12)

    Abstract:
    New applications in regenerative biotechnology require the ability to understand and control protein- surface interactions on micrometer and submicrometer length scales. Evidence presented here shows that micropatterned amphiphilic comb polymer films exhibit a pretreatment-dependent behavior with respect to protein adsorption for the proteins fibronectin, laminin, and for serum. A micropatterned surface, consisting of protein-reactive regions, separated by comb polymer, was created and tested for protein adsorption using the surface-sensitive imaging tool TOF-SIMS. Immersion of micropatterned surfaces in solutions of fibronectin or laminin resulted in uniform protein coverage on both the comb polymer and protein-reactive regions. However, preimmersion of similarly patterned surfaces in water for 2 h prior to protein incubation was found to dramatically improve the protein-resistant properties of the comb polymer regions. These results are consistent with poly(ethylene glycol) (PEG) side chain reorientation and/or hydration and poly-(methyl methacrylate) (PMMA) backbone segregation away from the interface region. © 2005 American Chemical Society.

    Keywords:
    Surface treatment;Proteins;Adsorption;Biotechnology;Molecular dynamics;Plastic films;Materials testing;Secondary ion mass spectrometry;Polyethylene glycols;Polymethyl methacrylates;Molecular orientation;Interfaces (materials);