Papers Published

  1. Dreher, M.R. and Elas, M. and Ichikawa, K. and Barth, E.D. and Chilkoti, A. and Rosen, G.M. and Halpern, H.J. and Dewhirst, M., Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry, Med. Phys. (USA), vol. 31 no. 10 (2004), pp. 2755 - 62 [1.1782677] .
    (last updated on 2007/04/12)

    Hyperthermia, as an adjuvant with radiation and chemotherapy, has shown promise in the treatment of cancer. The relevant biological effects of a hyperthermia treatment are both time and temperature-dependent, creating a need for accurate thermometry. We present a novel noninvasive thermometry modality that combines a temperature responsive biopolymer, the elastin-like polypeptide (ELP), and nitroxide to produce an ELP-nitroxide conjugate. When examined with electron paramagnetic resonance (EPR) spectroscopy, the ELP-nitroxide conjugate has temperature-dependent spectral line widths whose predictive accuracy is ~0.3°C (80 μM). We believe that the temperature-dependent changes observed in the EPR spectrum are due to the combined effect of temperature, viscosity and effective radius on the rotational correlation time of the ELP-nitroxide conjugate

    biochemistry;cancer;EPR line breadth;EPR spectroscopy;hyperthermia;proteins;tumours;