- Shimoboji, Tsuyoshi and Stayton, Patrick S. and Long, Cynthia and Chilkoti, Ashutosh and Chen, Guohua and Harris, J. Milton and Hoffman, Allan S., Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein,
Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium, vol. 1
pp. 897 - .
(last updated on 2007/04/12)
A novel method for sensitive environmental control of the molecular recognition process between a protein receptor and its ligand is presented. The method is based on the conjugation of a smart, temperature-sensitive polymer, poly(N-isopropylacrylamide), to a specific genetically-engineered site, thiol. It was shown that the site-specific conjugation of a temperature-sensitive polymer to a genetically engineered protein provides a very sensitive temperature control of the ligand-protein recognition process. Such molecular switches could have widespread applications in medicine and biotechnology.
Genetic engineering;Phase transitions;Thermal effects;Biotechnology;