- Meyer, Dan E. and Kong, Garheng A. and Dewhirst, Mark W. and Foulon, Catherine and Zalutsky, Michael R. and Chilkoti, Ashutosh, Genetically engineered polypeptide carrier for thermal targeting of anticancer therapeutics,
American Chemical Society, Polymer Preprints, Division of Polymer Chemistry, vol. 41 no. 1
pp. 1020 - 1021 .
(last updated on 2007/04/12)
The thermally-responsive carrier, an elastin-like polypeptide (ELP), is an oligomer of a Val-Pro-Gly-Xaa-Gly pentapeptide repeat. Below a specific, inverse phase transition temperature (Tt), ELPs are highly soluble in aqueous solution. However, upon raising the Tt, they undergo a reversible transition resulting in desolvation and aggregation. Control of the amino acid sequence and the molecular weight of the carrier by genetically encodable synthesis enables precise molecular design of ELPs with an inverse transition of -40 °C. ELP was studied whether it will remain soluble systematically but will accumulate at sites of externally-induced local hyperthermia where the temperature is raised above Tt.
Amino acids;Solubility;Phase transitions;Oligomers;Molecular weight;Genetic engineering;Synthesis (chemical);Hyperthermia therapy;Fluorescence;Microscopic examination;Molecular structure;